|
KMID : 1101420150470040313
|
|
Korean Journal of Clinical Laboratory Science
2015 Volume.47 No. 4 p.313 ~ p.317
|
|
|
Rapid Detection of Mycobacterium tuberculosis Complex in Tissues by Using the Nested PCR
|
|
Park Jung-Yeon
Yang Byoung-Seon
|
|
|
Abstract
|
|
|
|
Due to the increase in incidence of infection of Mycobacterium tuberculosis complex (MTC), it is imperative that a rapid diagnosis accompanies the handling of MTC. This is due to the three to eight weeks it takes to culture Mycobacteria, and the lack of sensitivity of microscopic examination of AFB. Recently, nested PCR has been used to detect and diagnose mycobacteria. It is especially useful in complementing diagnosis by histological extra pulmonary. After culturing all the specimens and practicing the nested PCR, we did comparison analysis between nested PCR and culture. There were 76 specimens, 31 of which were positive. Of the 31 positive specimens in culturing, only 22 were positive in nested PCR. Of the 45 negative specimens, 36 were negative in nested PCR. As a result, Sensitivity was 71% and specificity was 80%. Furthermore, the positive predictive value was 71% and negative predictive value was 80%. These results indicate that nested PCR based techniques are sensitive, specific, and rapid methods for the detection of MTC.
|
|
|
KEYWORD
|
|
|
Mycobacterium tuberculosis, Positive predictive value, Nested PCR
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|